By changing the Deflection , you can adjust the cantilever deflection voltage maintained by the feedback loop in Contact AFMImage mode. This parameter defines the center of the range of Cantilever Deflection Voltages that can be collected.
If the Data Center = 0, then the setpoint is at the center line of the force plot. Changing the setpoint shifts the force calibration curve on the graph. For example, if the setpoint is set to –3.0 V and the data center is 0, the cantilever deflection axis of the graph centers around –3.0 V. Raising the setpoint to –2.0 V shifts the force calibration curve down by one volt so the graph centers at –2.0 V. Changing the value of this parameter also changes it in Image mode.